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<title>Add sTCW Database</title>
<body style="padding:20px;width:650px;font-family:Times">
<h2>Add sTCW Database to multiTCW project</h2>

<h3>1. Select sTCW Database</h3>

Press this button to view a menu of available databases on the hosts
listed in HOSTS.cfg. (Click on a host to expand its database list). 
Select the desired database and press "Keep".

<h3>2. Prefix</h3>

Each sTCW database needs to have a unique prefix, which is used to prefix library names
in case two projects share library names. 

<h3>3. Generate protein file or Use existing</h3>

mTCW clustering is based on amino acid alignment, hence transcript nucleotide
sequences need to be converted to protein sequences. If you have already done
this, select "Use existing protein file", and browse to the file. 

<p>
Otherwise, select "Generate protein file" to have mTCW run ESTScan to extract
the amino acid sequences. ESTScan needs a training file ("SMAT file") in order to run. 
Click the file browse button to browse to and select the SMAT file. The browser 
should open to "embr.smat", which is supplied with TCW and is suitable for plants. 
For other projects you will need to create your own SMAT file, as described in the
online documentation. 
 
 <p>If the singleTCW database was built with protein sequences, this step is ignored.

